Fig. 3: Comparative analysis of luminescence decays of L-donor and S-donor undergoing TG-LRET to IRDye800 acceptor. | Nature Communications

Fig. 3: Comparative analysis of luminescence decays of L-donor and S-donor undergoing TG-LRET to IRDye800 acceptor.

From: Near-infrared long lifetime upconversion nanoparticles for ultrasensitive microRNA detection via time-gated luminescence resonance energy transfer

Fig. 3

a Schematic diagram illustrating the energy transfer of L-donor compared to S-donor, both with acceptor (IRDye800) bound to the surface. UCL decay curves of the (b) L-donor, (c) M-donor, and (d) S-donor as a function of acceptor concentration under a 980 nm short pulse (5 ns) excitation. UCL decay curves of the (e) L-donor, (f) M-donor, and (g) S-donor as a function of acceptor concentration under a 980 nm long pulse (5 ms). The changes in luminescence decay time values (-ΔτD) were compared for (h) the L-donor samples in (b) and (e), for (i) the M-donor samples in (c) and (f), and for (j) the S-donor samples in (d) and (g). L-donor, NIR long-lived luminescent UCNP donor; M-donor, NIR intermediate-lived luminescent UCNP donor; S-donor, NIR short-lived luminescent UCNP donor; TG-LRET, time-gated LRET; SS-LRET, LRET under steady-state excitation. Source data are provided as a Source data file.

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