Fig. 3: Nature of viremia, antibody and antigen-specific T cells induced by R3-UpA mutant in rats and mice. | Nature Communications

Fig. 3: Nature of viremia, antibody and antigen-specific T cells induced by R3-UpA mutant in rats and mice.

From: Design and nonviral delivery of live attenuated vaccine to prevent chronic hepatitis C virus-like infection

Fig. 3

A Viremia in R3-UpAhigh and RHV-rn1 infected rats (23 rats in each group) on 7 dpi. RHV-rn1 viremia data were compiled from our other studies using the same viral stock and from 8 rats used as controls in this study. Two-sided unpaired t-test was performed; p-value (****,  <0.0001). B Serum anti-NS3 IgG antibody titers after R3-UpAhigh infection in six vaccinated rats. C Specificities of antigen-specific T cells isolated from the liver of R3-UpAhigh infected rats (n = 3) at 14 dpi, three animals with their 3 replicates were shown as dots. Unpaired t-test and p-values between Media and NS3 (*, 0.0269), NS4 (***, 0.0006), NS5A (*, 0.0403), NS5B (*, 0.0429), Epitopes-Pool (*, 0.0358) respectively. Data points are presented as individual values and error bar at mean ± SEM. D Rat MHC class I tetramer incorporating an NS5B peptide was used to determine the frequencies of virus-specific CD8 T cells in the livers of R3-UpA vaccinated rats euthanized on 14 dpi. Representative data from three rats were shown using different colors. E Representative data showing the difference in phenotype of vaccine-primed memory CD8 T cells specific for NS5B peptide (red) and total CD8 (blue) T cells in the liver of vaccinated rats. F Viremia in R3-UpAhigh (n = 10) and RHV-rn1 infected mice on day 7 pi. Two-tailed unpaired t-test with p-value = (ns,0.1222). Data points were shown with mean ± SEM. G Frequencies of mouse MHC class I and class II specific CD8 and CD4 T cells in the liver (circle) and spleen (triangle) in R3-UpA and RHV-rn1 cleared mice (n = 5) on 45 dpi. Two-tailed unpaired t-test for Class I Tet+ response between R3-UpA Liver and RHV -Liver (**, 0.0084) and between R3-UpA Spleen and RHV-Spleen (*, 0.0356). Data points are presented as individual values and error bars at mean ± SEM. H Comparative analysis of total CD8 T cells in the liver and spleen with antigen-specific memory CD8 T cells in R3-UpA and RHV mice on 45 dpi. I Comparative analysis of total CD4 T cells in the liver and spleen with antigen-specific memory CD4 T cells in R3-UpA and RHV mice on 45 dpi. H and I use the same color scheme. Source data are provided as a Source Data file.

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