Fig. 2: Elevated neutrophil levels are associated with the negative behavioral sequelae of CSD stress. | Nature Communications

Fig. 2: Elevated neutrophil levels are associated with the negative behavioral sequelae of CSD stress.

From: Chronic social defeat stress induces meningeal neutrophilia via type I interferon signaling in male mice

Fig. 2

Plots show standardized effect sizes for neutrophil levels on behavioral outcomes shown in Fig. 1. Left to right: USM marking (nHC = 16, nCSD = 17. N = 5 experiments), SI social approaches (nHC = 22, nCSD = 26. N = 10 experiments), OF novel arena exploration (nHC = 21, nCSD = 23. N = 9 experiments), and L/D box crosses to light (nHC = 15, nCSD = 17. N = 7 experiments). Mixed generalized linear regression modeling with maximum likelihood estimation (MLE) revealed negative relationships between USM marking behavior and blood (**FDR = 0.0087, β = -5.12, z = -2.98, 95% CI [-4.47, -1.12]), iv- (*FDR = 0.016, β = -4.60, z = -2.55, 95% CI [-4.50, -0.85]), and iv+ (*FDR = 0.021, β = -4.74, z = -2.30, 95% CI [-4.74, −0.65]) meningeal neutrophils. Blood (**FDR = 0.0053, β = −2.59, t = −3.50, 95% CI [−0.82, −0.24]) and iv+ (*FDR = 0.020, β = −2.88, t = −2.61, 95% CI [−1.06, −0.13]) meningeal neutrophils are significantly associated with OF anxiety-like behavior. There were no significant relationships between neutrophils and SI or LD. See Tables S1–S4 for statistics. Unless otherwise indicated, ordinary least squares (OLS) regression was used for modeling. Significance levels reflect FDR-adjusted p values. LD light/dark, OF open field, SI social interaction, USM urine scent marking. *p < 0.05, **p < 0.01. Source data provided in Source Data file.

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