Fig. 5: CSD leads to widespread tissue increase in neutrophils, relationships present between skull and meningeal neutrophils support microscopy data.
a Flow cytometry shows increased neutrophil levels throughout the body following CSD stress in C57BL/6 J mice (REML model, main effect of group, ****p < 0.0001, F(1,15) = 112.8, nHC = 8, nCSD = 9; for blood, nHC = 7). See Table S9 for post-hoc statistics. b Unsupervised hierarchical clustering of data from (a) shows significant relationships between BM (Skull: ***FDR = 0.0005, r = 0.83, t(15) = 5.81. Tibia: **FDR = 0.009, r = 0.75, t(15) = 4.36) and iv- meningeal neutrophils, but not with blood. Blood and iv+ meningeal neutrophils were significantly correlated (*FDR = 0.018, r = 0.72, t(15) = 3.97). Asterisks in heatmap indicate significant Pearson correlation after Bonferroni correction for multiple comparisons. See Table S10 for correlation coefficients and FDR values. Data points represent individual mice; data represent 4 independent experiments. Multiple tissues were collected from individuals; repeat measures were accounted for in (a). BM = bone marrow, CSD = chronic social defeat stress, HC = home cage, iv = intravascular. Data shown as mean ± SEM. *p < 0.05, **p < 0.01, ****p < 0.0001. Source data are provided as a Source Data file.
