Fig. 3: Conserved salt bridges stabilize the Stomatin oligomer.
From: Structural basis for membrane microdomain formation by a human Stomatin complex
a Side view of the Stomatin 16-mer cage with subunits colored individually. The structure is divided into four layers (I–IV) based on height from the membrane interface. b Top-down view of the complex at layer I showing a salt bridge between E59 and R70 at the SPFH1 domain from neighboring subunits. c Layer II view showing a salt bridge between R205 (wall helix) and E262 (CapH) from subunits two positions apart, illustrating cross-subunit stabilization. d Layer III view highlighting interactions among E227 (wall helix), K220 (adjacent wall helix), and R251 (CapH from another subunit). e Layer IV view showing a salt bridge between E243 and K235 between neighboring subunits. Insets in (b–e) display zoomed-in views of representative salt bridges. Green and purple spheres mark residue positions. These salt bridges form a conserved electrostatic network critical for maintaining oligomer integrity and are enriched at positions associated with disease mutations in podocin.
