Fig. 5: αSyn conformation-dependent interaction with AK2, DJ1, and ATP synthase.

a Volcano plot shows LiP-MS results on peptides significantly changing upon mitochondrial treatment with αSyn fibrils or monomers for 30 min. Red dots show statistically significant hits (Fold Change > 1.5, q-value < 0.05, based on a two-tailed t-test followed by Benjamini–Hochberg adjustment for multiple hypothesis testing). LiP-MS hits peptides mapped to the protein structure when treated with αSyn monomer or fibrils for AK2 (b), ATP synthase (c), and DJ1 (d). The peptide changes found for AK2 were the same as with αSyn monomers, while for DJ1, only one of two regions found with monomers were also found with αSyn fibrils. Hits passing the q-value cutoff (<0.05) are shown in red. b, d, peptides significant only at a p-value cutoff (<0.05) are shown in pink. p-values were calculated using a two-tailed t-test. FDR adjustment was used for q-value determination. Source data are provided as a Source Data file.