Fig. 6: Influence of αSyn on ATP hydrolysis in cells.

a Whole cellular ATP content in mice HT-22 cells in the presence of WT and ΔC-αSyn monomers and fibrils. b ATP hydrolytic capacity in isolated mice liver mitochondria after the addition of increasing concentrations of WT and ΔC-αSyn monomers and fibrils. PBS treatment was used as a control, and its hydrolytic activity was set at 100%. Oligomycin A, a potent ATP Synthase inhibitor, was used as a positive control. Statistical significances were calculated with one-way ANOVA followed by Dunnett’s multiple comparisons test. ^****p-value < 0.0001, ^***p-value < 0.001, ^**p-value < 0.01, ^*p -value < 0.05. p-value ≥ 0.05 was considered non-significant. All data are given as mean ± SD. Experiments for each setup were performed on four separate days (a, b). In total, n = 10–12 replicates for (a) and n = 8–15 replicates for (b) were used for each condition. Exact p-values and n for each condition are provided in the Source Data file. Source data are provided as a Source Data file.