Fig. 2: CryoEM analysis of F-ENA fibers found on the surface of BtK spores. | Nature Communications

Fig. 2: CryoEM analysis of F-ENA fibers found on the surface of BtK spores.

From: Cryo-EM identifies F-ENA of Bacillus thuringiensis as a widespread family of endospore appendages across Firmicutes

Fig. 2

a 60k magnification cryoEM micrograph of an F-ENA fiber purified from Btk. b 2D class average of extracted F-ENA fiber segments. c Reconstructed cryoEM volume of F-ENA (color coded per fiber segment, which corresponds to an F-ENA trimer). d Reconstructed cryoEM volume and corresponding molecular model of S-ENA. e Cartoon representation of a single F-ENA protomer, which belongs to the DUF 4183 family characterized by two juxtaposed beta-sheets interconnected by a series of loops, and an N-terminal extension (Ntc) that serves as the docking mechanism for incoming F-ENA trimers to connect to the fiber terminus. f Consensus logo of the N-terminal hook derived from a multiple sequence alignment of the top 500 blast hits using WP_001121647.1 as the query (g) molecular model of F-ENA and top-view image of the F-ENA pilus (h) Zoom-in of the Ntc of segment i docked into the receiving hydrophobic groove of segment i-1. i Lateral stacking at the trimeric interface through a predominantly polar interaction with an interface area of 5680 Å2, involving seven identified hydrogen bonds. j Electrostatic (left) and hydrophobic (right) surface coloring. The image in (a) is a representative image from two cryoEM datasets that were collected from two biological replicates.

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