Fig. 3: Structural organization of the F-ENA. | Nature Communications

Fig. 3: Structural organization of the F-ENA.

From: Cryo-EM identifies F-ENA of Bacillus thuringiensis as a widespread family of endospore appendages across Firmicutes

Fig. 3

a Genetic locus of the genes associated with the biogenesis of F-ENA; HP, hypothetical protein. b X-ray crystal structure of ExsF (pink) in complex with a peptide (F-Anchor14-28) corresponding to residues 14–28 of F-Anchor (green). c Zoom-in of the ExsF/F-Anchor14–28 interaction. d AlphaFold3 (AF3) prediction of the F-ENA/F-Anchor complex—a truncated F-Anchor spanning residues 140–276 was used for clarity purposes. e Schematic representation of the F-type pili tethered at the spore-proximal end to the exosporium via a tripartite F-ENA/F-Anchor/ExsF complex, and decorated at the spore-distal terminus with the tip-fibrillum F-BclA. f X-ray crystal structure of the C-terminal head group (F-BclA727-863) of F-BclA, g AF3 prediction of the F-ENA/F-BclA1–46 complex with zoom-in of the docking interface.

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