Fig. 2: Recombination events observed in the mating progeny of Sssmr1-1 and Ssatg4. | Nature Communications

Fig. 2: Recombination events observed in the mating progeny of Sssmr1-1 and Ssatg4.

From: Normal meiosis in the fungus Sclerotinia sclerotiorum despite the irregular distribution of haploid chromosomes between two nuclei

Fig. 2

a Genetic dissection of mating between Sssmr1-1 and Ssatg4. The genotypes of Sssmr1-1 and Ssatg4 mutants are denoted as sR and Sr, respectively. At the Ssatg4 locus, R represents the WT allele and r is the mutant Ssatg4 allele. At the Sssmr1-1 locus, S represents the WT allele and s is the mutant Sssmr1-1 allele. Following mating, the resulting diploid fusion cell goes through meiosis and gives rise to four types of gametes with equal probability. These gametes produce progeny with three phenotypic classes, segregating in a 1:1:2 ratio (WT-like: Sssmr1-1-like: Ssatg4-like) according to the Mendelian law. b The relative positions of sscle_12g089570 (for SsATG4) and sscle_12g091490 (for SsSMR1) on Chromosome 12. The two gene loci are highlighted in cyan and red, respectively. c A summary table showing the markers and their presence in the colonies derived from ascospores resulted from the mating of Sssmr1-1 and Ssatg4. The phenotypes of the colonies are indicated as WT, Sssmr1-1, or Ssatg4-like in the table. The table includes chromosome number (Chrom), gene code, and single nucleotide polymorphism (SNP) in red or black, indicating markers derived from Sssmr1-1 or Ssatg4, respectively. Two gene codes connected by a dash (-) indicate that the SNP is located in the intergenic region between them. Genes with causal mutations in Sssmr1-1 and Ssatg4 are highlighted in bold. The number of reads from NGS are presented as two numbers in each cell, color-coded for clarity. The number before the comma represents the SNP counts of the indicated marker in the reference genome (WT S. sclerotiorum 1980), while the number after the comma represents the SNP counts of the indicated marker in the mutant genome. For all sequenced progeny, cyan indicates markers from Ssatg4, red indicates markers from Sssmr1-1, and white indicates no information. d Chromosome maps of selected progeny, as translated from the marker information from c. The chromosome regions containing only Ssatg4 markers are arbitrarily colored in cyan, extending either to the end of the chromosome or to the appearance of an Sssmr1-1 marker. Similarly, regions containing only Sssmr1-1 markers are arbitrarily colored in red, extending either to the end of the chromosome or to the appearance of an Ssatg4 marker. Chromosome regions with no marker information are shown in white. The maps were drawn using MapChart (v2.32). Note that the chromosome map does not fully represent the true physical arrangement due to the limitations in the density of available markers.

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