Fig. 3: The impact of siRNA-mediated depletion of GWL and B55α-δ on cellular responses to GWL inhibition by C-604.

a Representative images of RPE-1, HeLa and BT-549 cells treated with siRNAs targeting B55α, B55β, B55γ, B55δ or GWL exposed to C-604 for 48 h. Images from one of n = 3 experiments are shown. The scale bar represents 50 µm. b Distributions of cell cycle groups in control and C-604-treated populations depleted of B55α-δ or GWL. The results of n = 3 independent experiments are shown for each condition as separate columns. The cell cycle state in the stacked bar plot and indication of statistical significance are colour-coded as specified by the colour legend below the plots. Between 407 (Experiment 3, BT-549, siB55β, 2 µM C-604) and 32,941 (Experiment 3, HeLa, siB55β, 0 µM C-604) cells were analysed. Statistical significance of differences between proportions of 4 N and 8 N+ cells was determined by an unpaired two-tailed t-test. Parametric statistical testing was justified by the Shapiro-Wilk test of normality (p ≥ 0.46). Determined p-values were adjusted for multiple testing using the Benjamini-Hochberg procedure. ns – not significant, * p < 0.05, ** p < 0.01, *** p < 0.001.