Fig. 2: Task neurons in S1 and PPC areas.
From: Coordinated multi-level adaptations across neocortical areas during task learning
a Diagram of the cranial window and S1/PPC locations. Simultaneous two-photon calcium imaging was performed in S1 and PPC areas in GCaMP6f transgenic mice. b Example FOVs at 3 imaging depths from day 1 and day 20 of the same mouse, same area (PPC-RL). c Example neuronal activity from day 1 and day 20, from the same mouse and the same area as shown in (b). Colored stripes in the background represent task windows. Black lines represent ΔF/F traces, red lines represent deconvolved spike rates in arbitrary units. d Percentage of task-responsive neurons in S1, PPC-RL, and PPC-A. Colors represent the task window to which the neurons are responsive. e Percentage of task-discriminative neurons in S1, PPC-RL, and PPC-A. Colors represent the task window to which the neurons are responsive. f Session-average spike rates of task-discriminative neurons over trial time. Spike rates were z-scored within each session. Gray lines represent the naive condition, colored lines represent the expert condition. Dashed vertical lines indicate task windows. g Quantification of average spike rates of task-discriminative neurons, in different task windows. Colors represent the quantified task window. (S1: 13 mice, 130, 190, 153 sessions [naive, learning, expert]; PPC-RL: 13 mice, 70, 77, 104 sessions; PPC-A: 9 mice, 60, 111, 45 sessions; *p < 0.05, **p < 0.01, ***p < 0.001; Statistical tests in (d, e, g) were performed using two-sided Wilcoxon rank-sum test between Naive and Learning sessions, and between Naive and Expert sessions, using pooled session data; p-values were corrected for multiple comparisons using FDR method; mean ± SEM). Source data are provided as a Source Data file.
