Fig. 3: Identifying a pan-neuropathy cellular transcriptional response pattern.

A Differences in cluster abundance in patients with polyneuropathy (PNP) vs. controls (CTRL), left: main clusters (Fig. 1B), right: immune cell subclusters (Fig. 2A). Only clusters with a log₂ fold change > 0.5 are shown. B Correlations between individual single nuclei and PNP status (right), Inflammatory Neuropathy Cause and Treatment (INCAT) disability score (middle), and g-ratio of myelinated axons (right) are shown. Red indicates a positive, blue a negative correlation. C The number of differentially expressed genes (DEG) per cell cluster was determined in a pseudobulk approach and is visualized. D Neighborhood graph illustrating the number of DEG between PNP and CTRL in a cluster-free neighborhood-based approach. Each dot denotes a neighborhood. Edges reflect the number of cells shared between neighborhoods. The panel on the right displays a higher magnification of the neighborhoods with the largest number of DEGs. E Volcano plots of DEG in 0.1, and the vertical dashed lines display a log₂ fold change of 2. Selected transcripts above those thresholds are labeled. Statistical significance was assessed using a two-sided likelihood ratio test, with p-values adjusted for multiple comparisons using the Benjamini-Hochberg method.