Fig. 3: Characterization of meiotic RNPs containing 4E-T.
From: Translational repression by 4E-T is crucial to maintain the prophase-I arrest in vertebrate oocytes
a Xenopus stage-VI oocytes were lysed and treated with RNaseA as indicated. Lysates were subjected to immunoprecipitation with unspecific control (Ctrl) or 4E-TAb1 antibodies. Samples were immunoblotted as indicated. One representative experiment of three independent biological replicates is shown. Quantification of this figure can be found in Fig. S4a. b Xenopus stage-VI oocytes were injected with mRNA encoding Myc-LSM14A. 18 h after injection, oocytes were treated with PG and lysed at the indicated time points. Lysates were treated with RNaseA as indicated and subjected to immunoprecipitation with 4E-TAb1 antibodies. Samples were immunoblotted as indicated. One representative experiment of three independent biological replicates is shown. c Signals in IP samples from (b) were quantified and normalized to 4E-T signal in IP samples. Values were normalized to t = 0 h / −RNaseA condition and are given as mean±s.d. from three independent biological replicates. p values within –RNaseA and +RNaseA conditions were calculated using one-way ANOVA with Dunnett’s multiple comparisons test. d Xenopus stage-VI oocytes were injected with the indicated 4E-T or unspecific control (Ctrl) antibodies and mRNAs encoding Flag-TRIM21, Myc-PATL2 and Myc-LSM14A. 18 h after injection, oocytes were lysed and separated by sucrose density gradient centrifugation. Gradient fractions were analyzed by immunoblot as indicated. As reference, fractions containing 4E-T in Ctrl-depleted oocytes are shown as dashed rectangles in both conditions. Asterisk indicates IgG HC. One representative experiment of three independent biological replicates is shown. Source data including additional loading controls are provided as a Source Data file.
