Fig. 4: 4E-T directly interacts with eIF4E and PATL2 in immature Xenopus oocytes.

a Schematic representation of 4E-T.S from Xenopus laevis. Regions required for interaction with the indicated proteins in somatic cells are highlighted⁵³. b Xenopus stage-VI oocytes were injected with water or mRNA encoding the indicated Flag-4E-T^TRIM variant and a mixture of mRNAs encoding Myc-eIF4E1b, Myc-PATL2 and Myc-LSM14A. 18 h after injection, oocytes were lysed and subjected to immunoprecipitation with Flag antibodies. Samples were immunoblotted as indicated. One representative experiment of three independent biological replicates is shown. Quantification of this figure can be found in Fig. S5a. c Xenopus stage-VI oocytes were injected with water or mRNA encoding the indicated Flag-4E-T^TRIM variant. 18 h after injection, oocytes were lysed and subjected to immunoprecipitation with Flag antibodies. Samples were immunoblotted as indicated. One representative experiment of four independent biological replicates is shown. Quantification of this figure can be found in Fig. S5a. d Xenopus stage-VI oocytes were injected with water or mRNA encoding the indicated Flag-4E-T^TRIM variant and a mixture of mRNAs encoding Myc-PATL2 and Myc-LSM14A. 18 h after injection, oocytes were lysed and subjected to immunoprecipitation with Flag antibodies. Samples were immunoblotted as indicated. Asterisk indicates unspecific bands. One representative experiment of three independent biological replicates is shown. e Signals in IP samples from (d) were quantified. Signals in IP samples of water-injected oocytes were subtracted and values were normalized to Flag. All conditions were normalized to WT and values are given as mean±s.d. from three independent biological replicates. p values were calculated using one-way ANOVA with Dunnett’s multiple comparisons test. Source data including additional loading controls are provided as a Source Data file.