Fig. 4: CDX0239 is internalized and localizes to the lysosome in ALK-expressing neuroblastoma cell lines. | Nature Communications

Fig. 4: CDX0239 is internalized and localizes to the lysosome in ALK-expressing neuroblastoma cell lines.

From: A humanized anaplastic lymphoma kinase (ALK)-directed antibody-drug conjugate with pyrrolobenzodiazepine payload demonstrates efficacy in ALK-expressing cancers

Fig. 4

A Quantification of internalized Fabfluor-conjugated CDX0239 (blue line with squares) compared to Fabfluor-conjugated IgG control (gray line with squares) in ALK-amplified NB-1 and in ALK WT low surface-expression SK-N-AS plotted as total integrated density mean ± standard deviation over 48 h with n = 5 technical replicates for the IgG condition in NB-1 and n = 6 technical replicates for all other conditions. Sample size and datasets are located within the Source Data File. Source data are provided as a Source Data File. B Pictographs of the internalization of Fabfluor-conjugated CDX0239 compared to Fabfluor-conjugated IgG in NB-1 and SK-N-AS over 24 h. C Lattice light-sheet microscopy images of NB-1 and SK-N-AS cells treated with LysoSensor 3 h after treatment with Fabfluor-conjugated CDX0239 or Fabfluor-conjugated IgG with overlay. Represented data has been validated with at least 2 independent experiments.

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