Fig. 3: 5-HT or CP-93219 decreased the frequency of spontaneous EPSC (sEPSC) events. | Nature Communications

Fig. 3: 5-HT or CP-93219 decreased the frequency of spontaneous EPSC (sEPSC) events.

From: Serotonin and psilocybin activate 5-HT1B receptors to suppress cortical signaling through the claustrum

Fig. 3

A Top, representative sEPSC events before and after exposure to 10 μM 5-HT for 10 min. Middle left, 10 μM 5-HT did not alter sEPSC amplitude (two-tailed paired Student’s t-test, t7 = 2.18, p = 0.066, n = 8 neurons from 4 animals). Middle right, cumulative probability plot of sEPSC amplitude before (gray) and after (blue) exposure to 10 μM 5-HT. Bottom left, exposure to 10 μM 5-HT increased the interevent interval (IEI) of sEPSCs (two-tailed paired Student’s t-test, t7 = 2.55, p = 0.038, n = 8 neurons from 4 animals). Bottom right, cumulative probability plot of sEPSC IEI before (gray) and after (blue) exposure to 10 μM 5-HT. B Top, representative sEPSC events before and after exposure to 10 μM CP-93129 for 10 min. Middle left, 10 μM CP-93129 did not alter sEPSC amplitude (two-tailed paired Student’s t-test, t7 = 0.21, p = 0.84, n = 8 neurons from 6 animals). Middle right, cumulative probability plot of sEPSC amplitude before (gray) and after (blue) exposure to 10 μM CP-93129. Bottom left, 10 μM CP-93129 increased the interevent interval (IEI) of sEPSC events (two-tailed paired Student’s t-test, t7 = 2.56, p = 0.037, n = 8 neurons from 4 animals). Bottom right, cumulative probability plot of sEPSC IEI before (gray) and after (blue) exposure to 10 μM CP-93129. C Experimental diagram corresponding to D and E. D Representative a-oEPSCs elicited by stimulation of ACC terminals at baseline and after exposure to 10 μM CP-93129 for 10 min. E Top, 10 μM CP-93129 did not alter a-oEPSC amplitude (two-tailed paired Student’s t-test, t11 = 0.057, p = 0.96, n = 12 neurons from 4 animals). Bottom, 10 μM CP-93129 increased interevent interval of a-oEPSCs elicited by ACC terminal activation (two-tailed paired Student’s t-test, t11 = 2.30, p = 0.042, n = 12 neurons from 4 animals). Scale bars are 5 s (horizontal) by 20 pA (vertical) for A and B, top. Scale bars are 20 ms (horizontal) by 40 pA (vertical) for D. All error bars represent SEM. Source data are provided as a Source Data file.

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