Fig. 3: Localization of Rrp42-EL.
From: 4D structural biology–quantitative dynamics in the eukaryotic RNA exosome complex
PRE effects of Rrp42C59S, A106C-TEMPO on Ile-δ1 of (A) Csl4 in Exo9, (B) Rrp41 in Exo9 (red) and Exo10 (light red), (C) Rrp45 in Exo9 (orange) and Exo10 (light orange) without RNA and (D) Rrp45 in Exo9 (orange) and Exo10 (light orange) with RNA. n.d.: value not determined due to signal overlap. Data were obtained from one measurement. Error bars are derived from the signal-to-noise ratio in the NMR spectrum and represent ±1 SD. Ile-δ1 PREs of (E) Csl4 (in Exo9), (F) Rrp41 (in Exo10) and (G) Rrp45 (in Exo10) plotted onto the cryo-EM structure. Csl4 is shown in blue, Rrp41 in red, Rrp45 in orange and Rrp42 in green. Note, that Rrp42-EL is not visible in the structure (see Fig. 1C). For clarity only the outline of the subunit that is NMR active is shown. Residues that show strong PREs are labeled.
