Fig. 4: Dynamics of Rrp42-EL.
From: 4D structural biology–quantitative dynamics in the eukaryotic RNA exosome complex
A19F spectra of Rrp42C59S, A106C-TFA in Exo9 without (top, purple) and with (bottom, pink) RNA and (B) corresponding RD profiles. C Inversion recovery experiments (curves) and 19F 1D spectra (insets) for Rrp41D113tfmF in Exo9 Rrp42C59S, A106C-TEMPO without (left) and with (right) RNA for paramagnetic (in color) and diamagnetic (in black) samples. (D) 19F spectra of Rrp42C59S, A106C-TFA in Exo10 without (top, teal) and with (bottom, light teal) RNA and (E) corresponding CEST profiles, acquired for B1 = 25 Hz. F Inversion recovery experiments (curves) and 19F 1D spectra (insets) for Rrp41D113tfmF in Exo10 Rrp42C59S, A106C-TEMPO without (left) and with (right) RNA for paramagnetic (in color) and diamagnetic (in black) samples. G, J Schematic depiction of Rrp42-EL dynamics in Exo9 (G) without and (H) with RNA and in Exo10 (I) without and (J) with RNA. Rrp41 is in red, Rrp45 in orange, Csl4 in blue and Rrp42 in green. The green loop depicts Rrp42-EL, the green dot A106CTFA/TEMPO and the red dot Rrp41D113tfmF. Fits for panels C and F were obtained using Eq. 1. Fits for panels B and E were obtained using the model described in the methods. Error bars in B, C and F are derived from the signal-to-noise ratio in the NMR spectrum and represent ±1 SD. Errors in G and I are ±1 SD and obtained as described in the “Method section”.
