Fig. 6: Rrp42-EL blocks an aberrant RNA access path.
From: 4D structural biology–quantitative dynamics in the eukaryotic RNA exosome complex
RNA activity assays for (A) wtExo10 (wt, gray crosses, solid line) and Exo10 Rrp42Δ93-125 (Δ, yellow circles, dashed line) and (B) for channel-blocked Exo10 Rrp45-L (L, gray crosses, solid line) and Exo10 Rrp45-L Rrp42Δ93-125 (L-Δ, yellow circles, dashed line). The lines are global linear fits to the linear activity regime. For an extended analysis of alternative linear regimes see Supplementary Fig. 26. C Catalytic activity of exosome constructs. P values are derived from a two-sided, paired-sample t-test. wt = wtExo10, Δ = Exo10 Rrp42Δ93-125, L = Exo10 Rrp45-L, L-Δ = Exo10 Rrp45-L Rrp42Δ93-125. Data were obtained from three biological replicates with three technical repeats. Error bars represent ±1 SD. D Rrp42-EL allows on-path RNA to access Rrp44 for degradation (left) but blocks a direct access path towards Rrp44 (right).
