Fig. 1: Tn-seq screen reveals that disruption of motV increases V. cholerae infant mouse colonization.
From: Vibrio cholerae motility is associated with inter-animal transmission
10 postnatal day 4 Crl:CD1(ICR) mice (henceforth P4 CD1) were intragastrically inoculated with ~5 × 107 CFU of a mariner transposon library. 18 h later, the SIs were homogenized and pooled, and after overnight growth on LB plates, transposon insertion sites were determined by sequencing (pooled litter). For comparison, the same library was passaged overnight on LB plates (LB library). n = 1 pooled litter of 10 pups and 1 LB plate. A Volcano plot comparing the fold-change in gene insertion frequency between the pooled litter and LB library with P value derived from a two-sided Mann-Whitney test. Colonization factors are genes with P value < 0.01 and log2 fold-change <−2. Data for the tcp operon (toxin-coregulated pilus) and flagellar assembly genes (categorized from KEGG database vch02040) are expanded in (B). C Transposon insertion frequency across the genome highlighting increased insertion frequency in cheR-2, cheY-3, and motV from the pooled intestinal samples. Tn-seq data are available in Supplementary Data 1. Source data are provided as a Source Data file.
