Fig. 6: Or/In-Se NPs reverses ischemic stroke by triggering exogenous antioxidation and endogenous selenoprotein activation.
From: Organic-inorganic covalent selenium reversing ischemic reperfusion injury
a Schematic illustration of dual action mechanisms of Or/In-Se NPs in the treatment of ischemic stroke. Organic part of Or/In-Se NPs scavenges ROS to H2O for direct exogenous antioxidation, while the inorganic Se transforms to selenoproteins to exhibit endogenous antioxidation. The icons of brain tissues are created with BioRender.com. b mRNA expression level of selenoproteins in brain tissue on the injured side of MCAO mice after treated by Or/In-Se NPs. Concentration for antioxidative enzyme of (c) SelP, (d) Gpx, (e) TrxR and (f) MDA in brain tissue homogenate of the injured side (mean ± SD, n = 3 biologically independent samples, One-Way ANOVA test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, compared with saline group). Immunofluorescence images of (g) ALOX15, (h) Gpx4 and (i) TfR1 expression in the damaged brain of MCAO mice with different treatments. j H&E staining, (k) Nissl staining, (l) NeuN expression and (m) TUNEL staining images of damaged brain tissues in MCAO mice with different treatments, n = 3 biologically independent samples. The red arrow in (k) represents a normal neuron. Source data are provided as a Source Data file.
