Fig. 5: SPIDER SVI-generated pattern results on single-cell-resolution Slide-SeqV2 mouse brain dataset.

A The cell type annotation of the sample. B The boxplot of the correlations between the activation strength and the profiles of member and non-member SVIs in each pattern shows that the activation strength represents the member SVIs (n=163 LRIs). C Deconvoluted cell types and their corresponding patterns are displayed, with annotated correlation coefficients for each pattern. For each pattern, two member SVIs having the highest correlation coefficients with the activation strength are plotted. D Top ten enriched pathways for genes contained in each SVI pattern, under the significance threshold of adjusted p-value ≤ 0.05. E Cell clustering using SVI patterns. F Cell type compositions in SVI pattern-based clusters. Clusters having a cell type with a majority of over 30% are displayed. G–I Clusters involved in the cell types and their top three marker SVIs. G Oligodendrocytes. H CA1/CA2/CA3 subiculum. I Astrocytes. Corr: Pearson correlation coefficient. The boxplots display the median (center line), the 25 and 75th percentiles (box bounds), whiskers extending to the most extreme data points within 1.5 × the interquartile range, minima and maxima as the lowest and highest points within the whiskers, and outliers as individual points beyond the whiskers. The statistical significance of box plots is calculated using one-sided Mann-Whitney-Wilcoxon test with Benjamini-Hochberg correction, with the exact adjusted p-values listed in Supplementary Table 7 and the following significance annotations: ****: adjusted p-value ≤ 1.00e-04; ***: 1.00e-04  < adjusted p-value ≤ 1.00e-03; **: 1.00e-03  < adjusted p-value ≤ 1.00e-02; *: 1.00e-02  < adjusted p-value ≤ 5.00e-02. Source data are provided as a Source Data file.