Fig. 1: Leaf-specific abundant amentoflavone-type biflavonoids and its unique dimerase GbCYP90J6 in G. biloba. | Nature Communications

Fig. 1: Leaf-specific abundant amentoflavone-type biflavonoids and its unique dimerase GbCYP90J6 in G. biloba.

From: Gymnosperm-specific CYP90Js enable biflavonoid biosynthesis and microbial production of amentoflavone

Fig. 1

a Detailed schematic of plant tissue sampling. YL young leaf, uML unmature leaf, ML mature leaf, YS young shoot, OS old shoot, OR old root, MR main root, FR fibrous root. b Dry weight content of amentoflavone (1), bilobetin (2), ginkgetin (3), and isoginkgetin (4) in selected tissues of G. biloba. All data represent the means values ± SD; error bars show the standard deviation of three biological replicates. Source data are provided as a Source Data file. c Expression patterns of screened peroxidases and CYPs in different organs. The figure displays the values as log2(average FPKM of biological replicates). d LC-MS/MS analysis of tobacco leave crude extract expressing the chr1.3004 (GbCYP90J6) candidate with the filtration of substrate apigenin (5). The empty vector was used as a negative control. The MS2 spectra of the 1 standard (black) and the product from GbCYP90J6-expressing tobacco (blue) were shown on the right. e The dimeric reaction catalyzed by GbCYP90J6 with 5 as substrate.

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