Fig. 3: MUSHER activates DOG1 expression during secondary dormancy induction in response to high temperature. | Nature Communications

Fig. 3: MUSHER activates DOG1 expression during secondary dormancy induction in response to high temperature.

From: Chromatin retained MUSHER lncRNA integrates ABA and DOG1 signalling pathways to enhance Arabidopsis seeds dormancy

Fig. 3

a Seed germination of WT and different musher mutants after 7 days of secondary dormancy (SD) induction at 35 °C. Germination frequency was scored after 5 days. Data are presented as mean values of four biological replicates +/− SD. *p < 0.05 (t-test two-sided). b Seed germination of WT and cpl1-7 mutant seeds after 7 days of SD induction. Germination was scored after 5 days. Data are presented as mean values of five biological replicates +/− SD. *p < 0.05 (t-test two-sided). c The expression profile of DOG1 was analysed using RT-qPCR in seeds of WT, msh-1 and msh_dCas9-1 subjected to secondary dormancy induction. Data are presented as mean values of four biological replicates +/− SD. UBC21 mRNA was used as a reference gene. d The DOG1 relative expression level in seeds of WT and cpl1-7 mutant, subjected to SD induction. Data normalised to UBC21 and related to WT. Data are presented as mean values of four biological replicates +/− SD. p-value from the two-tailed t-test, *p < 0.05. e DOG1 and f MUSHER expression profile in after-ripened seeds subjected to secondary dormancy induction at 25 °C and 35 °C. Expression was normalised to UBC21 mRNA and dry seed levels. Data are presented as mean values of four biological replicates +/− SD (*p < 0.05, two-sided t-test). g Schematic representation of constructs introduced to transgenic plants. RT-qPCR analysis of luciferase mRNA levels in seeds subjected to secondary dormancy induction under temperatures indicated on the right. LUC expression level measured after 5 days of SD induction, normalised to UBC21 and related to 25 °C for each construct, Data are presented as mean values of four biological replicates +/− SD (*p < 0.05, two-sided t-test). h Long and short DOG1 expression analysis on the 5th day of secondary dormancy induction in WT at temperatures indicated. Data are presented as mean values of four biological replicates +/- SD (p-value from the two-tailed t-test, *p < 0.05). UBC21 mRNA was used as a reference gene.

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