Fig. 3: In vivo CRISPR screen of Cryptosporidium vaccine candidates.

a Mouse faecal material was collected and luminescence was monitored during infection. Each replicate was conducted with 2 KO vectors per gene. Data shows the mean faecal luminescence from a pooled cage sample ( ± SEM of 2 technical replicates), n = 5 Ifnγ^–/– mice for screen 1 and n = 3 Ifnγ^–/– mice for screen 2. b Rank ordered fold enrichment scores from the vaccine candidate CRISPR screens. The colour indicates the relative fitness contribution of a gene, with dark purple being high fitness conferring and dark green being low fitness conferring. c Comparison of the fold enrichment scores between the replicate CRISPR screens for each gene. Confidence refers to the inverse of the 95% confidence interval when comparing the log2 fold change scores from each screen (see “Methods”). d Mouse faecal material was collected and luminescence was monitored during infection. Data shows the mean faecal luminescence from a pooled cage sample ( ± SEM of 2 technical replicates), n = 5 Ifnγ^–/– mice. Barcodes from KO parasites could be easily monitored over time (e) and within individual mice (f).