Fig. 1: Schematic of MELISSA workflow.
From: Modeling integration site data for safety assessment with MELISSA
MELISSA receives as input 1. IS data in a bed file format, 2. a design matrix with all the relevant samples’ annotation, 3. a reference genome or a bed file containing genomic region to be tested for IS enrichment. MELISSA combines input data and performs two main types of analysis: gene targeting rates and gene/clone fitness alterations. MELISSA also has a dedicated function for calculating clonality indexes, graphical representation of the results, and downstream pathway enrichment analyses.
