Fig. 2: Conditional hepatic deletion of G3BP1 exacerbates HFD-induced MASLD. | Nature Communications

Fig. 2: Conditional hepatic deletion of G3BP1 exacerbates HFD-induced MASLD.

From: Dysregulation of GTPase-activating protein-binding protein1 in the pathogenesis of metabolic dysfunction-associated steatotic liver disease

Fig. 2

A Liver gross morphology of wild-type (WT) and G3BP1 LKO mice fed normal chow (NC) or high-fat diet (HFD) for 20 weeks (n = 6), imaged by Nikon SMZ25 stereomicroscope. Liver weight (B) and liver-to-body weight ratio (C) of WT and G3BP1 LKO mice fed normal chow (NC) or HFD for 20 weeks (n = 6 mice per group). All values are presented as means ± S.E.M. Statistical analysis was performed using two-way ANOVA followed by Sidak’s multiple comparisons test (two-sided). *P < 0.05; **P < 0.01. D H&E-stained liver sections from WT and G3BP1 LKO mice fed NC or HFD for 20 weeks (n = 6), imaged using Leica microscope. Scale bars: 50 µm. E Representative images of liver sections stained with Oil red staining from G3BP1 LKO and WT mice that were fed NC or HFD for a duration of 20 weeks (n = 6 mice per group). Scale bars, 50 µm. The right panels depict the percentage of Oil red positive area of liver sections from indicated groups, analyzed using ImageJ. Data are presented as means ± S.E.M. (n = 6 mice per group). Each dot represents the average value from 10 images collected per mice. ****P < 0.0001 by two-way ANOVA followed by Sidak’s post hoc test for multiple comparisons (two-sided). F Glucose tolerance tests (GTT) were performed in G3BP1 LKO and WT mice after NC or HFD consumption, and the corresponding AUC values were calculated (n = 6 mice per group). All values are presented as means ± S.E.M. Statistical analysis was performed using two-way ANOVA followed by Sidak’s post hoc test for multiple comparisons (two-sided). Exact p values are shown in the figure. *: WT-NC vs. WT-HFD; #: LKO-HFD vs. WT-HFD. G Insulin tolerance test (ITT) assays data for the non-model control and MASLD model G3BP1 LKO and WT mice, and the AUC (area under the curve) values were calculated accordingly (n = 6 mice per group). All values are presented as means ± S.E.M. Statistical analysis was performed using two-way ANOVA followed by Sidak’s post hoc test for multiple comparisons (two-sided). Exact p values are shown in the figure. *: WT-NC vs. WT-HFD; #: LKO-HFD vs. WT-HFD. Hepatic total cholesterol (TC, H) and triglycerides (TG, I) quantified by enzymatic colorimetric assays in WT and G3BP1 LKO mice under NC or MASLD model induced by HFD for 20 weeks (n = 6 mice per group); All values are presented as means ± S.E.M. ****P < 0.0001, analysis of two-way ANOVA followed by Sidak’s post hoc test for multiple comparisons (two-sided). Serum alanine aminotransferase (ALT, J) and aspartate aminotransferase (AST, K) concentrations quantified by enzymatic assays in WT and G3BP1 LKO mice under NC or MASLD model induced by HFD for 20 weeks (n = 6 mice per group); All values are presented as means ± S.E.M. ****P < 0.0001, analysis of two-way ANOVA followed by Sidak’s post hoc test for multiple comparisons (two-sided).

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