Fig. 4: Cryo-EM of bYFVES504/DIII17D and bYFVAsibi/DIII17D.
a Cryo-EM density map of bYFVES504/DIII17D with I3 symmetry applied and coloured according to its local resolution. b Cryo-EM reconstruction and atomic model of the bYFVES504/DIII17D ASU. c Cryo-EM density map of bYFVES504/DIII17D:2C9 with I3 symmetry applied and coloured according to its local resolution. d Cryo-EM reconstruction and atomic model of bYFVES504/DIII17D:2C9 ASU. e Cryo-EM density map of bYFVAsibi/DIII17D with I3 symmetry applied and coloured according to its local resolution. f Cryo-EM reconstruction and atomic model of bYFVAsibi/DIII17D ASU. In (e) and (f) the pentagons, triangles, and ovals represent the icosahedral five-, three- and two-fold axes, respectively. In (b), (d), and (f) the density map was obtained via symmetry expanded ASU reconstruction using cisTEM2 and is shown in either grey (E) or blue (2C9 Fab), with the atomic models shown in red (E-DI), yellow (E-DII), blue (E-DIII) and grey (2C9 Fab). g IC50 values of recombinant hIgG1 anti-YFV or anti-flavivirus mAbs against the bYFV DIII chimeras. Neutralisation was determined via FRNTs on C6/36 (Aedes albopictus) cells. Lines indicate group medians, and each symbol represents a technical replicate from three biological replicates. For bYFV17D/DIIIAsibi and bYFVAsibi/DIII17D against 5A n = 3, and for all other groups n = 6. Parental bYFV17D and bYFVAsibi controls are from Fig. 1E. bYFV DIII chimeras were compared to parental viruses by two-tailed Mann-Whitney tests on GraphPad Prism 9. **p ≤ 0.002. For 2A10G6: p = 0.002 (17D vs 17D/DIIIAsibi) and p = 0.004 (Asibi vs Asibi/DIII17D), 6B6C−1 and 864: p = 0.002 (17D vs 17D/DIIIAsibi and Asibi vs. Asibi/DIII17D). LOD = limit of detection. Source data is provided as a Source Data file.
