Fig. 2: Design and in vitro characterization of the OK-MLIR system.
From: Dual-key cooperatively activated DNA regulator for controlling mitochondria-lysosome interactions
a Schematic illustration of the structure and light triggered OK-MLIR binding with mitochondrion. b Native PAGE (10 wt%) analysis of the assembly and light-triggered activation of OK-MLIR. Lanes 1–6 represent: DNA ladder marker, B1, B2, CD63APT-TPP, OK-MLIR, and OK-MLIR with 10-min UV light treatment, respectively. c Confocal fluorescence images of HeLa cells transfected without (Blank) and with CD63APT-TPP, CD63APT or R1-TPP (180 nM). MitoRed and FAM were excited with 579 nm and 494 nm lasers, respectively. Scale bar: 25 µm. d Pearson Correlation analysis of (c) by investigating the fluorescence signals of FAM and mitochondria (MitoRed). Shown are mean ± standard error the mean (SEM) from ten individual cells. (CD63APT-TPP vs CD63APT, P = 6.01 × 10−13 and CD63APT vs R1-TPP, P = 8.44 × 10−12, ****P < 0.0001, two-tailed Student’s t test). e Confocal fluorescence images of HeLa cells treated without (Blank), and with CD63APT, OK-MLIR, or OK-MLIR (180 nM) in presence of 10-min UV irradiation. LysoTracker and FAM were excited with 580 nm and 494 nm lasers, respectively. Scale bar: 10 µm. f Pearson Correlation analysis of (e) by investigating the fluorescence signals of LysoTracker and FAM. Shown are mean ± SEM from ten individual cells. (OK-MLIR vs OK-MLIR (UV), P = 9.74 × 10−11, ****P < 0.0001, two-tailed Student’s t test). Image representation of 3 experiments. Source data are provided as a Source Data file.
