Fig. 4: Design and performance of DK-MLIR in living cells.
From: Dual-key cooperatively activated DNA regulator for controlling mitochondria-lysosome interactions
a Schematic illustration of the structure and dual keys triggered DK-MLIR activation for mitochondrial binding. b Confocal images of lysosomes (LysoTracker) and mitochondria (MitoTracker Green) in HeLa cells treated without (Blank) and with 180 nM DK-MLIR (the DK-MLIR row), 180 nM DK-MLIR in presence of 100 µM BSO and 10-min UV irradiation (BSO + UV), 180 nM DK-MLIR in presence of 100 µM BSO (BSO), and DK-MLIR in presence of 10-min UV irradiation (UV). MitoTracker Green and LysoTracker were excited with 490 and 580 nm lasers, respectively. Scale bar: 50 µm. c Pearson Correlation analysis was performed on the red and green fluorescence in (b). Shown are mean ± SEM from ten individual cells. (Blank vs UV, P = 1.72 × 10−13, ****P < 0.0001, two-tailed Student’s t test). d Confocal immunofluorescence images of HeLa cells treated without (Blank) and with 180 nM DK-MLIR (DK-MLIR), 180 nM DK-MLIR in presence of 100 µM BSO and 10-min UV irradiation (BSO + UV), 180 nM DK-MLIR in presence of 100 µM BSO (BSO), and DK-MLIR in presence of 10-min UV irradiation (UV); and then labeled with COXIV antibody (green) and DAPI (blue). Scale bar: 60 µm. e Green fluorescence intensity was extracted from (d) for quantitative analysis. Shown are mean ± SEM from ten individual cells. (Blank vs UV, P = 6.90 × 10−16, ****P < 0.0001, two-tailed Student’s t test). f Western Blot map of TOMM20 and COXIV proteins in HeLa cells treated with 180 nM DK-MLIR in presence of 100 µM BSO (Lane 1), 180 nM DK-MLIR (2), 180 nM DK-MLIR in presence of 10-min UV irradiation (3), and 180 nM DK-MLIR in presence of 100 µM BSO and 10-min UV irradiation (4). Image representation of 3 experiments. Source data are provided as a Source Data file.
