Fig. 4: Transcriptional expression of fungal CAZymes in carbohydrate degradation across non-polar and polar oceans.
From: Organic matter degradation by oceanic fungi differs between polar and non-polar waters
Top 81 fungal CAZymes identified through random forest classification (A), their corresponding abundance profiles (B), and the activity levels of secreted CAZymes (C). Feature importance of the top 81 CAZymes identified using random forest classification (ntree = 500), with feature types distinguishing between non-polar and polar oceans. Statistical significance of each variable’s importance (Mean Decrease Accuracy) was assessed using the rfPermute package in R113. P-values were derived from permutation-based tests (two-sided), with significance levels indicated in Supplementary Data 7. No correction for multiple comparisons was applied. Model accuracy was assessed by measuring the decrease in performance when each variable was excluded, while the mean decrease in the Gini coefficient reflects the contribution of each variable to the homogeneity of nodes and leaves in the random forest results. Heatmap illustrating the expression of top 81 CAZymes and their targeting of various biogeochemically important polysaccharides. The relative abundance of CAZyme classes was log2(n + 1) transformed. The complete random forest results can be found in Supplementary Data 7. Source data are provided as a Source Data file.
