Fig. 1: Interaction between Dab2320–495 and the individual EH domains of Eps15. | Nature Communications

Fig. 1: Interaction between Dab2320–495 and the individual EH domains of Eps15.

From: Promiscuous and multivalent interactions between Eps15 and partner protein Dab2 generate a complex interaction network

Fig. 1

Schematic illustration of (A) Eps15 and (B) Dab2. The disorder prediction (IUPred2A23) is shown as a black curve along the sequence. Above horizontal line: predicted disordered, Below horizontal line: predicted ordered. The folded domains are shown as gray boxes above the disorder prediction. Putative interaction motifs are shown as colored circles along the sequence (legend on the right of Dab2). DLL/DLF: clathrin binding; DPF/DPW: AP2α binding; FxDxF: AP2β binding; NPF: EH domain binding. The stretches used in this study are boxed with gray dashed lines. C Zoom into a 1H−15N HSQC spectrum of Dab2320–495 in the absence and presence of EH2 (left) and EH1 (right). D–F CSPs of 100 μM Dab2320–495 in the presence versus the absence of increasing concentrations of EH1 (D), EH2 (E) and EH3 (F), respectively. Color legends are displayed in the respective plots. Filled pink circles denote positions of phenylalanines.

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