Fig. 4: Interaction between EH123 and Dab2_320–495.

A ¹⁵N R_1ρ spin relaxation of Dab2_320–495 (100 µM) in the absence and presence of increasing concentrations of EH123 at a ¹H frequency of 600 MHz. B CSPs between the ¹H-¹⁵N HSQC of ¹⁵N Dab2_320–495 in the absence and presence of increasing amounts of EH123. The filled pink dots represent the phenylalanines present in the Dab2_320–495. C ¹⁵N R_1ρ spin relaxation of the individual ¹⁵N EH domains (EH1, EH2, EH3) as well as EH123 in the absence and presence of 100% Dab2_320–495 at a ¹H frequency of 600 MHz. D CSPs between the ¹H-¹⁵N TROSY-HSQC of ¹⁵N EH123 in the absence and presence of increasing amounts of the Dab2_320–495. The color legends are indicated in the respective figure panels. The relaxation rates in (A) and (C) were derived from a fit of peak intensities against the relaxation delay. Errors of the fitted rates were derived from the experimental uncertainty.