Fig. 5: Astrocyte-specific proteomes reveals cytosolic changes by silencing Ca2+ signaling.
From: Prefrontal cortex astrocytes modulate distinct neuronal populations to control anxiety-like behavior
a Schematic of the astrocyte cytosolic-TurboID approach and experimental procedure. b IHC images showing strong streptavidin signals localized with astrocyte marker S100β in the mPFC (n = 3 mice for control and n = 3 mice for Astro-cyto-TurboID). c Venn diagram depicting the numbers of proteins identified in control and CalEx mPFC astrocytes. d Left, protein network analysis map of the top 100 most abundant common proteins. Node size and color represent protein expression fold change between CalEx and control. Edges represent protein association network from the STRING analysis. Right, networks of proteins highlighted for key biological processes of astrocytes. e Volcano plots showing unique and common astrocyte proteins. Differentially expressed common proteins are separated by dotted lines (P < 0.05). f Heatmap showing differentially regulated proteins with a fold change greater than 2, with annotation of primary functions. g Heatmap showing the abundance (average peak area) of selected unique proteins related to GPCRs, neurotransmitter and ion transport, synapse regulation, Ca2+-dependent vesicle release, cell adhesion and lipid metabolism. h Gene Ontology (GO) terms for significant biological pathways of unique proteins detected in mPFC astrocytes. Two-sided two sample t-tests were used for all statistical comparisons of the proteomic data. Source data are provided in Supplementary Data 1.
