Fig. 6: Astrocyte-neuron interface proteomes identifies cell-specific changes.
From: Prefrontal cortex astrocytes modulate distinct neuronal populations to control anxiety-like behavior
a Schematic of the split-TurboID approach. b IHC images showing streptavidin signals associated with split-TurboID expression (n = 2 mice for Astrocyte-C-TurboID and n = 3 mice for Astrocyte-C-TurboID + Neuron-N-TurboID). c Venn diagram depicting the numbers of interface proteins identified in control and CalEx mPFC samples. d Volcano plots showing unique and common interface proteins. Differentially regulated proteins are separated by dotted lines (P < 0.05). e Venn diagram depicting the numbers of identified cell type-specific interface proteins. f–h Protein network analysis maps of differentially regulated interface proteins and unique interface proteins expressed in astrocytes (f) excitatory neurons (g) and inhibitory neurons (h) with proteins highlighted for biological processes with high confidence scores. Node size and color represent protein expression fold change. Edges represent protein association network from the STRING analysis. i Heatmaps showing the protein abundance (average peak area) and functions of interface proteins that are specifically identified in astrocytes, excitatory neurons, and inhibitory neurons. j Chord diagram of interaction pairs between interface proteins identified in astrocytes, excitatory neurons, and inhibitory neurons. k Heatmaps showing enrichment of identified cytosolic and interface proteins across various diseases associated with prefrontal dysfunction. OCD, obsessive-compulsive disorder; ASD, autism spectrum disorders; PTSD, post-traumatic stress disorder; ADHD, attention-deficit/hyperactivity disorder; BPD, borderline personality disorder; AD, Alzheimer’s disease; MS, multiple sclerosis. Two-sided two sample t-tests were used for all statistical comparisons of the proteomic data. Source data are provided in Supplementary Data 2.
