Fig. 5: Population- and single-molecule-level phenotypic characterization of single datA, DARS1 or DARS2 deletions in slow growth regime.

A Diffusion coefficient histograms of DnaA obtained from sptPALM with cells growing in slow growth regime in the absence of either datA, DARS1 or DARS2. Histograms are fitted (black line) with a theoretical description of 250,000 particles moving between a free and a bound state. The number of cells and tracks per histogram is shown in the upper left corner. On top, the kinetic rates describing the fitting are provided for each growth regime. B Average bound fraction of DnaA, as estimated from the kinetic rates, for wild-type, triple deletion and the three single deletion E. coli mutants. Data are presented as mean values ± SD, derived mathematically from kinetic rates in (A). P-values are calculated by a two-sided T test. C, D Comparison of the average number of origins of replication (C) and the cell area of the population (n_ΔdatA = 3016 cells, n_ΔDARS1 = 760 cells, n_ΔDARS2 = 1300 cells) (D). See Supplementary Fig. 6A for a complete list of replicates of DNA content measurements via flow cytometry. For bar graphs, data are presented as mean values ± SD, n = 3 biologically independent replicates. Box plots indicate median (middle line), 25th and 75th percentile (box) and 5th and 95th percentile (whiskers) as well as outliers (single points). P-values are calculated by a two-sided T test. Source data are provided as a Source Data file.