Table 1 Quantification of MT binding affinity for wild-type and mutated augmin N-clamp complexes from different species as determined using tubulin co-sedimentation assays

H. sapiens^wild-type

0.8

0.4

0.94

5

X. laevis^wild-type

0.6

0.4

0.91

3

A. thaliana^wild-type

0.01

0.09

0.89

3

D. melanogaster^wild-type

0.4

0.3

0.90

4

D. melanogaster^Msd5-GST

0.04

0.01

0.90

3

H. sapiens^HAUS8-ΔNT

–

–

–

3

X. laevis^HAUS8-ΔNT

2.2

2.1

0.69

3

A. thaliana^AUG8-ΔNT

2.1

1.5

0.89

3

D. melanogaster^{Dgt4- ΔNT}

2.9

0.5

0.99

3

H. sapiens^HAUS6-4A-mut

5.6

1.8

0.98

3

D. melanogaster^Dgt6-3A-mut

25.5

31.9

0.94

3

D. melanogaster^{Msd5- Δ81–93}

1.0

1.0

0.61

3