Fig. 4: Inhibition of vSub → VMH circuit attenuated FO-induced hypophagia.
From: Prolonged exposure to food odors suppresses feeding via an olfactory bulb-to-hypothalamus circuit
a Scheme for virus injection in the vSub and VMH of WT mice. b Validation of the expression of Arch in the vSub. Representative trace showing action firing potential changes in VMH-projecting vSub neurons with yellow light stimulation (c) and quantification of the membrane potential (d) and firing frequency (e). n = 5 neurons per group. f Three-hour chow intake from 6:30 pm to 9:30 pm in mice in Ctrl (no odor) or chow-derived odor exposure group with yellow or blue light stimulation. n = 7 mice per group. g Schematic figure for experimental procedure and odor exposure paradigm. Three-hour chow intake from 6:30 pm to 9:30 pm with saline or CNO treatment upon non-FO or FO exposure in chow-fed male mice with hM4Di expression in vSub-innervated VMH (h), LS (i), and NAcSh (j) neurons. n = 8 mice in (h, i); n = 10 mice in (j). Paired two-tailed t-tests in (d, e). Two-way ANOVA with post hoc Sidak’s multiple comparisons in (f, h, i, j). *P < 0.05, **P < 0.01, ***P < 0.001. Data are presented as mean ± SEM with individual data points. Source data are provided as a Source data file. The Schematics in (a) were created in BioRender. He, Y. (2025) https://BioRender.com/2gcdnui.
