Fig. 6: Constant activation of vSub-projecting OB neurons suppresses food intake and reduces body weight.
From: Prolonged exposure to food odors suppresses feeding via an olfactory bulb-to-hypothalamus circuit
a Scheme for virus injection in the vSub and OB of WT mice. b Representative images of virus expression in the OB. Representative trace of action potential firing by current injections in a step increment of 5 pA from resting membrane potential in the control (c) and NaChBac-expressing (d) neurons. e The minimum membrane potential required to induce action potential firing in control and NaChBac-expressing neurons in the OB. n = 6 neurons in Ctrl group and 5 neurons in NaChBac group. f The action potential width in control and NaChBac-expressing neurons in the OB. n = 8 neurons per group. Body weight change on chow diet (g), bi-daily chow intake (h) in the control and NachBac-expressing mice. n = 11 mice per group. Body weight on HFD (i) and weekly HFD intake (j) in the control and NachBac-expressing mice. n = 11 mice per group. Fat mass (k) and lean mass (l) in the control and NaChBac-expressing mice. n = 11 mice per group. Unpaired two-tailed t-tests in (e, f, k, l). Two-way Repeated Measures ANOVA with post hoc Sidak’s test in (g–j). Effect of NaChBac in (h), F(1, 20) = 4.574, #P < 0.05; Effect of NaChBac in (j), F(1, 20) = 27.44, ###P < 0.001; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data are presented as mean ± SEM with individual data points in (e, f, k, l) and as mean ± SEM in (g–j). Source data are provided as a Source data file. The Schematics in (a) were created in BioRender. He, Y. (2025) https://BioRender.com/jlynxc8.
