Fig. 3: Adsorbed protein on GA-lipo preserves targeting function.
From: Galloylated liposomes enable targeted drug delivery by overcoming protein corona shielding
a ζ potential of protein-adsorbed GA-lipo is dependent on the adsorbed protein type. (n = 3 technical replicates, mean value ± s.d.). b Super centrifugation was used to detect the adsorbed antibodies on the surface of GA-lipo. (pI: Bovine Serum Albumin, BSA 4.7, transferrin, TRF 5.4, Human hemoglobin, HBB, 6.8, Trastuzumab, TRA 8.7, Trypsin 10.1). Data are represented as the MFI of FITC-positive nanoparticles. (n = 3 technical replicates, mean value ± s.d.). c Structures of galloyl acid-modified cholesterol lipids with different lengths of PEG linker and schematic representation of GA-lipo and their capacity to adsorb proteins. d Trf adsorbed onto different GA-Chol modified liposomes. Data are represented as the MFI of FITC-positive nanoparticles. (n = 3 technical replicates, mean value ± s.d.). Statistical significance was analyzed by one-way ANOVA and Tukey’s multiple comparisons test. e Trf Trp quenching recovery after T@GA-lipo incubation with 100 mM Triton X-100, SDS, NaCl and Urea for 4 h. AUC normalized to folded TRA. (n = 3 technical replicates, mean value ± s.d.). f The result of incubating 4T1 cells with Trf@GA-lipo (5 μg/ml DOX) for 4, 8, 12, 24 h with 10% FBS. The concentration of uptake cells is shown. (n = 3 biologically independent samples, mean value ± s.d.). Statistical significance was determined by a two-way analysis of variance with Tukey’s multiple comparisons test. g The tumor accumulation of Trf@GA-lipo. (5 μg/kg DOX, n = 3 biologically independent samples, mean value ± s.d.). Significant differences were determined by two-way ANOVA and Tukey’s multiple comparisons test. Source data are provided as a Source Data file.
