Fig. 4: Move-up CmoKARI1 improves cucumber chilling tolerance by inducing isoleucine biosynthesis.
a Phenotypes, and b relative electrolyte permeability (REP) and MDA content of CmoKARI1-GFP root-transformed cucumbers after 12 h of 4 °C cold stress. a, b Each treatment includes 5 individual plants as n = 5 biological replicates (mean ± s.d., one-way ANOVA followed by Duncan’s test). Lowercase letters indicate significant differences (p < 0.05). c Phenotypes, and d REP and MDA in two-leaf-old cucumber seedlings treated with 300 μM L-isoleucine before and after 12 hours of chilling conditions. The control group received H2O. c, d At least n = 3 replicate pools were included, with each pool containing 12 plants, at both 0-h and 12-h chilling conditions (mean ± s.d., one-way ANOVA followed by Duncan’s test). Lowercase letters on the graphs indicate significant differences (p < 0.05). e Phenotypes, f REP and MDA content, g isoleucine content, and h Relative expression of CsaCBF1 and CsaCOR in two-leaf-old Super::CmoKARI1-GFP transgenic (TG) lines (TG#2 and TG#4) and wild-type cucumber seedlings before and after 12 h of chilling conditions. Cucumber ACTIN7 was used as an internal reference. The control group received H2O. Each treatment includes n = 3 (f, h), n = 5 (g) biological replicates, and each replicate includes 12 individual plants (mean ± s.d.), for each condition (one-way ANOVA followed by Duncan’s test for the 28 °C and 4 °C groups). Source data are provided as a Source Data file.
