Fig. 3: SEC-SAXS reveals a significant shape difference between CaMKIIα containing exon 14 or exon 18.
From: A domain-swapped CaMKII conformation facilitates linker-mediated allosteric regulation
A, B Size-exclusion chromatographs of CaMKIIα exon 14 and exon 18 are shown. The protein was monitored with absorbance at 280 nm as shown in black trace. Evolving factor analysis (EFA) indicates the main eluent component under the peak, which is shown for exon 14 (blue) and exon 18 (red). C, D SAXS profiles of CaMKIIα exon 14 and exon 18. Insets are the Guinier analysis to interpolate the Rg value ± SD of the fit for each construct. E Pair distance distribution of SAXS profiles of CaMKIIα exon 14 and CaMKIIα exon 18 were calculated in GNOM program in RAW v.2.1.4. Source data are provided as a Source Data Fig. 3.
