Fig. 4: The relationship between phenotype variation and severity can be observed in opercle-branchiostegal ray (op-br) complexes.
From: A quadratic paradigm describes the relationship between phenotype severity and variation
a Nine op-br complexes are shown for each genotype. Three severity classes are shown—low severity (wild types), mid severity (unselected homozygous mef2caco3008 mutants), and high severity (selectively bred high-penetrance homozygous mef2caco3008 mutants19,20,33). These animals were stained with Alizarin Red at 6dpf and imaged by whole-mount confocal microscopy. b Variance of op-br complex area was calculated for each severity class. Area variance was calculated among all op-br complexes imaged. A two-sided F-test was used to test equality of variances, and p-values are indicated (*≤0.05 p = 0.039, ***≤0.001 p = 0.0003). c Within-individual variation was also plotted by taking the absolute value of the difference in area between left and right op-br complexes. d Variances were calculated for all area shape features and plotted on a box and whisker plot. Average variance is higher in the mid-severity strain than in the other two strains. The p-values from a Dunnett T3 test are indicated (****≤0.0001). For (c) and (d) box and whisker plots, the box covers the 25th to 75th quartiles, the line is at the median, and whiskers extend to the minimum and maximum values. Source data are provided as a Source Data file.
