Fig. 6: Neural activities during flickering were predictable from sleep and decreased tuning stability.

a Using the pre-detour sleep probability transition matrix to predict spike activities during detour run flickering representations. b Example showing decoding result at fine time scale during detour run. Vertical gray lines mark theta peaks. Purple shaded areas are Mob-representing epochs (non-local) and red shaded areas are Det-representing epochs (local) during active run (> 10 cm/s). c Probabilities of spiking activities predicted by pre-run sleep during Mob- and Det-representing epochs (Left) and probability percentiles compared with shuffle sequences (Right). Wilcoxon rank-sum test. (n = 215 for mobile-representing epochs; n = 5345 for detour-representing epochs). d Using pre-reversal sleep probability transition matrix to predict spike activities during post-detour reversal run flickering representations. e Example showing decoding result at fine time scale during post-detour reversal run (shaded area highlighted local/non-local representing epochs). f Probabilities of spike activities predicted by pre-run sleep during RS- and Det-representing epochs (Left) and probability percentile compared with shuffle sequences (Right). Wilcoxon rank-sum test. (n = 1169 for reversal-representing epochs; n = 384 for detour-representing epochs). g Examples showing decoding result at fine time scale during post-detour run. The activities of 4 example pyramidal cells are highlighted. h Left, example showing pyramidal cells activities during detour-representing epochs and RS-representing epochs. Right, example showing distribution of detour-representing and RS-representing cells. i Spatial tuning curves of 4 highlighted example cells in (g) and (h) during pre-detour, detour, and post-detour sessions. Peak firing rates are marked for all the sessions. j Within session (post-detour) across-laps place map stability and across-sessions (pre-detour vs. post-detour) place map stability of detour- and RS-representing cells. Wilcoxon signed rank test. Bar plots in (j) display mean ± s.e.m. with each dot representing one animal, direction, and detour session (n = 20). Violin and box plots in (c) and (f) displayed the data distribution with whiskers representing the 5th to 95th percentile range; the box represented the 25th to 75th percentiles, and the center dot indicated the median value. ***P < 0.001, **P < 0.01, *P < 0.05. Source data are provided as a Source Data file.