Fig. 5: Aβ plaque staining in brain slices with LS7.
A Histological staining of the brain slices from the hippocampus region and the cortex region of WT or APP/PS1 mice using ThS (λex = 405 nm; λem = 415−500 nm) and LS7 (λex = 458 nm; λem = 555−735 nm), respectively. Scale bar: 50 μm. The experiment was repeated three times independently with similar results. B Co-localized images and coefficients of Fig. 5D. C The intensity profiles of ThS (black line) and LS7 (red line) in the linear regions of interest (ROI) in (A). D Merged images of ThS and LS7 channels, fine image of Aβ plaque under magnification, where green for the ThS and red for LS7. Scale bar: 10 μm.
