Fig. 8: CZP@LC promote osteogenesis and protection against cellular senescence due to mitochondrial dysfunction. | Nature Communications

Fig. 8: CZP@LC promote osteogenesis and protection against cellular senescence due to mitochondrial dysfunction.

From: Remodeling adipocytes’ lipid metabolism with a polycation loaded enzyme-active framework reverses osteoporotic bone marrow

Fig. 8

A Schematic diagram illustrating the experimental procedure. B–D Expression of osteogenesis-related genes (Ocn, Osx, and Runx2) in MC3T3-E1 cells after different treatments (n = 3 independent experiments). E–H Representative images and quantitative analyses of ALP and ARS staining (n = 5 independent experiments). I TEM images of the effect of BMAd-generated lipids on the mitochondria of MC3T3-E1 cells (yellow arrows point to mitochondrial cristae). J–M Representative images and quantitative analyses of JC-1 and mitoSOX staining (n = 5 independent experiments). N‒Q Images and quantification of different groups of MC3T3-E1 cells after DHE and SA-β-gal staining (n = 5 independent experiments). R–T Immunofluorescence costaining images and quantitative analysis of OCN and p21 in femoral sections (n = 5 mice). The data were expressed as the means ± SDs. Statistical significance was determined by one-way ANOVA. *p < 0.05, **p < 0.01, and ***p < 0.001.

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