Fig. 2: Network analysis reveals virus interactions with putative host protein complexes.

a Hub-and-spoke network showing 441 high-confidence interactions from the EBOV BioID2 screen. Gray circles represent proteins identified as unique hits using both GFP and other viral proteins as controls. Circles with thicker borders are 64 proteins identified with both N- and C-terminal tagged constructs. b PCSF interrelates BioID hits with the HIPPIE human protein-protein interaction (PPI) network. The algorithm connects hits via shortest pathways defined by the PPI network, considering interaction confidence and the presence of other hits. Hits are shown as green circles, where node size reflects BioID score. Two paths are shown connecting scored hits A and B. The lower path is shorter but less supported by literature citations and requires adding two non-hit proteins (Steiner nodes, triangles). The upper path is longer, includes more screen hits, and has higher-confidence edges with more literature support. The algorithm preferentially includes the upper path in the final solution. c Left Rescoring of hits identified by more than one viral protein. Host proteins were partitioned according to each viral protein’s contribution to the total SAINT score. Right Functional enrichment of subnetworks. Clusters identified by edge-betweenness (red and purple dashed circles), then subjected to gene set enrichment. d Full network generated by PCSF linking BioID2 hits through HIPPIE interactions. Colored circles indicate putative virus-host protein interactions. Lines connecting circles represent human PPIs. Five mRNA decapping complex proteins, each labeled by VP35, are highlighted in the inset. e Network annotated with pathways from Enrichr. Subnetworks are color-coded and numbered. Functional enrichments are in Supplementary Data 8. Source data are provided as a Source Data File.