Fig. 5: EBOV VP35 interacts with the C-terminal domain of EDC4.

a Schematic of EDC4 showing binding sites for DPC1A and DCP2. In the lower panel, lines indicate constructs used in co-immunoprecipitation and overexpression experiments. b Identification of EDC4 subdomain responsible for VP35 interaction. The indicated HA-tagged EDC4 constructs were co-expressed with FLAG-tagged VP35, and immunoprecipitation was performed with anti-HA antibody. One representative replicate of two biological replicates is shown. c EBOV RNA levels were measured by RNAFISH (magenta) after overexpression of the indicated EDC4 domains. Cell nuclei were stained with Hoechst 33342 (blue). Statistical significance was calculated by one-way ANOVA with multiple comparisons. P-value = * 0.0178; ****P-value < 0.0001. Six technical replicates of one biological replicate are shown. Data are presented as mean ± SD. d Transfection efficiency of EDC4 constructs as determined by HA staining (green). Scale bars = 100 μm. The right panel shows the proportion of cells stained with HA antibody. Six replicates of one biological replicate are shown. Data are presented as mean ± SD. e Cells were challenged with EBOV and fixed 48 hpi, then stained with antibodies against VP35 (grey) to label infected cells, EDC4 (red), and DDX6 (green). Both EDC4 and DDX6 are markers of P-bodies. Cell boundaries are indicated by dashed lines. Scale bar = 25 μm. One technical replicate of a representative biological replicate is shown. Source data are provided as a Source Data File.