Fig. 2: CRISPR knockout of PFDN promotes RV replication.

a Western blot detection of PFDN3 in knockout (PFDN3 KO) and wild-type (WT) HEK293 cells. b Western blot detection of PFDN4 in knockout (PFDN4 KO) and WT HEK293 cells. c WT, PFDN3 KO, and PFDN4 KO HEK293 cells were infected with RV (UK strain, MOI  =  5), and viral NSP5 mRNA level was measured at 24, 48, and 72 hpi by RT-qPCR. Data represents the average of three experiments; error bars indicate SEM (two-way ANOVA with Tukey’s multiple comparisons test). d WT, PFDN3 KO, and PFDN4 KO HEK293 cells were infected with RV (UK strain, MOI  =  5), and viral titers were measured at 1, 8, 24, 48, and 72 hpi by an FFU assay. Data represents the average of three experiments; error bars indicate SEM (two-way ANOVA with Dunnett’s multiple comparisons test). e WT, PFDN3 KO, and PFDN4 KO HEK293 cells were infected with RV (WI61 strain, MOI  =  5), and viral NSP5 mRNA level was measured at 72 hpi by an FFU assay. Data represents the average of three experiments; error bars indicate SEM (one-way ANOVA with Dunnett’s multiple comparisons test). f WT, PFDN3 KO, and PFDN4 KO HEK293 cells were infected with RV (OSU strain, MOI  =  5), and viral NSP5 mRNA level was measured at 72 hpi by an FFU assay. Data represents the average of three experiments; error bars indicate SEM (one-way ANOVA with Dunnett’s multiple comparisons test).